We further characterized CD31 lowCD45 low (29%) and CD31 highCD45 + (12%) cell populations, and the control cell population (37.3%) (Fig. Furthermore, the analyses revealed a CD31 −CD45 + cell population that we decided to use as a control (CTRL). CD31 +CD45 + cell populations are thought to be EMP progeny. A flow cytometry analysis of the CD31 +CD45 + Lin − cell population identified two discernible cell populations: CD31 low and CD31 high co-expressing CD45 low and CD45 +, respectively (Fig. Our aim was to achieve a prospective characterization of CD31 +CD45 + cell populations derived from Lin − cells. 1a supplemental material) to identify other cell surface markers that correlate with CD31 +CD45 + expression in adult peripheral blood. To evaluate whether some human erythrocytes in the adult could arise from existing CD31 +CD45 + circulating cells, we used a Lin −-enriched population derived from human peripheral blood (Fig. demonstrated that CD31(CD31/PECAM-1) is expressed on mouse HSCs throughout ontogeny and an erythroid progenitor cell population subset persists in adult bone marrow. A recent study indicated that EMPs persist until adulthood, and were found to be a source of endothelial cells. EMPs are found in a population of cells expressing CD31 and CD45 markers (CD31 +CD45 +). After birth, human erythropoiesis occurs in the bone marrow in specialized hematopoietic stem cell niches enriched in mesenchymal cells and other stromal cells. During embryogenesis, transient primitive hematopoiesis occurs in the yolk-sac, followed by another transient hematopoietic process, which starts from the erythro-myeloid progenitors (EMPs), the earliest precursors of erythrocytes, megakaryocytes, and macrophages, which originate in the yolk sac and migrate to the fetal liver. ![]() HSPCs are generated from a specialized population of endothelial cells, called hemogenic cells, in the aorta-gonad-mesonephros (AGM) region, through an endothelial-to-hematopoietic transition (EHT). ![]() Understanding the dynamic evolution of HSPCs will shed further light on erythropoiesis. Evolving views of hematopoietic stem and progenitor cell (HSPC) differentiation highlight complex regulation of lineage progression from highly heterogeneous HSPC populations.
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